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FRTL growing culture

FRTL growing culture

Item no.	CLS-550202
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	FRT-L, FR-TL, Fischer Rat Thyroid in Low-serum
Available
Growth properties	Adherent
Age	6 weeks
Gender	Unspecified
Breed	Fischer
Biosafety Level	1
Culture Medium	Coons F12
Medium Supplements	5% FBS
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Fluid Renewal	3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
Freeze Medium	CM-1 (CLS catalog number 800150)
Tumorigenic	No
Split Ratio	A ratio of 1:3 to 1:5 is recommended
Products	Thyroglobulin
Karyotype	Diploid
Manufacturer - Citation	FRTL (CLS catalog number 500202)
Rat_D1Wox31	104
Rat_D2Wox37	150
Rat_D19Wox11	212
Rat_D10Wox8	266
Rat_D4Wox7	153
Rat_D2Wox27	211
Rat_D5Rat33	136
Rat_D10Wox11	165
Rat_D1Wox23	210
Rat_D12Wox1	402
Rat_D6Wox2	112, 116
Rat_D8Wox7	182
Rat_D6Cebr1	233
SRY	X, Y

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

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Delivery expected until 8/21/2025

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