« Back
Home /
COS-7 growing culture

COS-7 growing culture

Item no.	CLS-665470
Manufacturer	CLS Cell Lines Service
Amount	1 cell culture flask
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	Cos-7, COS7, Cos7, CV-1 in Origin Simian-7
Available
Manufacturer - Applications	Transfection host. Suitable for transfection by vectors requiring expression of SV40 T antigen.
Growth properties	Monolayer, adherent
Cell type	Fibroblast
Age	Adult
Gender	Male
Morphology	Fibroblast-like
Biosafety Level	1
Culture Medium	DMEM:Ham' s F12
Medium Supplements	5% FBS, 2 mM L-glutamine
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
Fluid Renewal	2 - 3 times per week
Freezing Recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze Medium	CM-ACF (CLS catalog number 800650)
Split Ratio	A ratio of 1:4 to 1:8 is recommended
Seeding Density	1 x 10^4 cells/cm^2 will yield in a confluent layer in about 4 days
Virus Susceptibility	SV40 (lytic growth), SV40 tsA209 at 40Â°C, SV40 mutants with deletions in the early region
Products	T antigen
Manufacturer - Citation	COS-7 (CLS catalog number 605470)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 1 cell culture flask

available

Delivery expected until 5/29/2025

Express Delivery: Save 3 days

Compare

Add to wishlist

Recommend by mail