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COS-7 growing culture

COS-7 growing culture

Manufacturer	CLS Cell Lines Service
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	other
Amount	1 cell culture flask
Item no.	CLS-665470
eClass 6.1	32160890
eClass 9.0	32160890
Available
applications	Transfection host. Suitable for transfection by vectors requiring expression of SV40 T antigen.
age	Adult
gender	Male
morphology	Fibroblast-like
cellType	Fibroblast
growthProperties	Monolayer, adherent
citation	COS-7 (CLS catalog number 605470)
biosafetyLevel	1
virusSusceptibility	SV40 (lytic growth), SV40 tsA209 at 40Â°C, SV40 mutants with deletions in the early region
products	T antigen
cultureMedium	DMEM:Ham' s F12
mediumSupplements	5% FBS, 2 mM L-glutamine
subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
splitRatio	A ratio of 1:4 to 1:8 is recommended
seedingDensity	1 x 10^4 cells/cm^2 will yield in a confluent layer in about 4 days
fluidRenewal	2 - 3 times per week
freezingRecovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
freezeMedium	CM-ACF (CLS catalog number 800650)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cell culture flask

available

Delivery expected until 5/23/2024