CG055 Anti-Myc tag Monoclonal Antibody 5A5 (ChIP grade)

A myc tag is a polypeptide  protein tag  derived from the  c-myc  gene product that can be added to a protein using  recombinant DNA  technology. It can be used for  affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an  antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by  Western blotting.

The peptide sequence of the myc-tag is   N-EQKLISEEDL-C, where N stands for  Amino-terminus  and C stands for  Carboxy terminus. The tag is approximately 1202  Daltons  in atomic mass and has 10 amino acids.

It can be fused to the  C-terminus  and the  N-terminus  of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the  secretory pathway.

Monoclonal   5A5 was selected by its excellent   performance in   ChIP analyses.