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IEC-6 Cells European Partner

Item no. CLS-302149
Manufacturer CLS Cell Lines Service
Amount 1 ea
Certificate The certificate of analysis can be requested on the website or via email at info@cytion.com. Please indicate the lot number of your product in the email.
Specific against Rat (Rattus norvegicus)
Dry ice Yes
Alias IEC 6, IEC6, Intestinal Epithelioid Cell line No. 6
Available
Manufacturer - Applications
Transfection. Gene expression studies
Manufacturer - Category
Rat cell lines
Description
IEC-6 is an epithelial cell line derived from the rat small intestine, specifically the crypt cells. These cells are non-tumorigenic and have been instrumental in studies related to intestinal epithelial function, differentiation, and the mechanisms underlying intestinal diseases. IEC-6 cells retain the characteristics of normal intestinal epithelial cells, including the ability to differentiate and maintain contact inhibition. This cell line is particularly valuable for research focused on gastrointestinal biology, including the study of the effects of growth factors, cytokines, and various pharmacological agents on the intestinal epithelium.

IEC-6 cells are widely used in investigations of the cellular processes involved in intestinal regeneration and repair, making them essential in the study of gastrointestinal pathologies such as inflammatory bowel disease (IBD), and cancer. The cells are sensitive to growth inhibition by transforming growth factor-beta (TGF-β), which is commonly used to study the signaling pathways involved in epithelial cell proliferation and differentiation. Additionally, IEC-6 cells are utilized in research related to nutrient absorption and barrier function, helping to elucidate the role of the intestinal epithelium in maintaining gut homeostasis.
Tissue
Small intestine
Growth properties
Adherent
Cell type
Epithelial cell
Age
18-24 days
Gender
Male
Breed
Charles River Sprague Dawley (CD(SD))
Morphology
Epithelial-like
Biosafety Level
1
Culture Medium
DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Medium Supplements
Supplement the medium with 10% FBS
Subculturing
Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Freeze Medium
Handling of Cryopreserved Cultures

Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

Upon receipt, either store the cryovial immediately at temperatures below -150° C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

For immediate culturing, swiftly thaw the vial by immersing it in a 37° C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility
Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
Safety Precautions
When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
Disclaimer
Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting.
Warranty
We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 ea
Available: In stock
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