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Mouse Anti-6His IgG: unconjugated;100ug

Mouse Anti-6His IgG: unconjugated;100ug

Item no.	COL-00-1711-100ug
Manufacturer	Columbia Biosciences
Amount	100 ug
Category	Antibodies Primary Antibodies
Type	Antibody
Format	Liquid
Applications	WB, ELISA
Clone	AD1.1.10
Specific against	other
Host	Mouse
Isotype	IgG1
Conjugate/Tag	Unconjugated
Citations	Conrath, K. et al. (2001) Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs. J. Biol. Chem. 276: 7346-7350. Suen, J.L. et al. (2001) Characterization of self-T-cell response and antigenic determinant of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice. Immunol. 103: 301-309. Hoffmann, S.C. et al. (2007) Identification of CLEC12B, an inhibitory receptor on myeloid cells. J. Biol. Chem. 282: 22370-22375. Zheng, J. et al. (2007) Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen. BMC Immunol. 8: 4. Bahi, A. & Dreyer, J.L. (2008) Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization. Genes Brain Behav. 7: 244-256. Wrighton, K.H. et al. (2009) Transforming Growth Factor beta can stimulate Smad1 phosphorylation independently of Bone Morphogenic Protein receptors. J. Biol. Chem. 284: 9755-9763. Diefenbacher, M. et al. (2011) The Dsl1 Tethering Complex Actively Participates in Soluble NSF (N-Ethylmaleimide-sensitive Factor) Attachment Protein Receptor (SNARE) Complex Assembly at the Endoplasmic Reticulum in Saccharomyces cerevisiae. J Biol Chem. 286: 25027-38.Alvarez, M.M. et al. (2010) Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method. PLoS One. 5: e10176. Bahi, A. et al. (2008) The role of tissue-type plasminogen activator system in amphetamine-induced conditional place preference extinction and reinstatement. Neuropsychopharmacology. 33: 2726-34. Gunnarsen, K.S. et al. (2010) Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA. BMC Biotechnol. 10: 8. Hwang, H.Y. et al. (2008) Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment. Mol Immunol. 45: 2570-80. De Vooght, L. et al. (2012) Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly. Microb Cell Fact. 11: 23.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Manufacturer - Targets	6His
Clonality	Monoclonal
Description	Mouse anti-6x Histidine IgG, clone AD1.1.10, primary antibody, monclonal, unconjugated, 100ug
Immunogen	polyhistidine (HHHHHH) tagged polypeptide
Storage	4
Storage Buffer	100 mM Sodium Phosphate pH 7.4, 100 mM Sodium Chloride, 2 mM Sodium Azide
Dilution	Western blot analysis: 1:500-1:1000; IHC: 1:250-750
Reactivity	Polyhisitidine tagged proteins (N- or C-terminus label) Dilutions: Western blot analysis: 1:500-1:1000; IHC: 1:250-750
Form / Storage	5mg/ml in 100 mM sodium phosphate (pH 7.4), 150 mM sodium chloride, 0.1% BSA as a stabilizer, and 0.05% sodium azide
Stability	Maintain at +2-8°C for 12 months. Stable for 1 year. Avoid repeated freeze-thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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COL-00-1711-100ug-datasheet.pdf

COL-00-1711-100ug-safety-datasheet.pdf