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Streptavidin: SureLight™ APC kinase assay format;1mg

Streptavidin: SureLight™ APC kinase assay format;1mg

Item no.	COL-D3-2212-K01-1mg
Manufacturer	Columbia Biosciences
Amount	1 mg
Category	Flow Cytometry Reagents & Chemicals Staining & Dyes Fluorescent Reagents
Type	Reagents
Format	Lyophilized
Applications	FC
Specific against	other
Conjugate/Tag	APC
Citations	Auclair, D., Miller, D., Yatsula, V., Pickett, W., Carter, C., Chang, Y., et al. (2007). Antitumor activity of sorafenib in FLT3-driven leukemic cells. Leukemia (Vol. 21). Bader, B., Butt, E., Palmetshofer, A., Walter, U., Jarchau, T., & Drueckes, P. (2001). A cGMP-dependent protein kinase assay for high throughput screening based on time-resolved fluorescence resonance energy transfer. J Biomol Screen (Vol. 6). Bamborough, P., Angell, R. M., Bhamra, I., Brown, D., Bull, J., Christopher, J. A., et al. (2007). N-4-Pyrimidinyl-1H-indazol-4-amine inhibitors of Lck: Indazoles as phenol isosteres with improved pharmacokinetics. Bioorganic & Medicinal Chemistry Letters (Vol. 17). Barrios Sosa, A. C., Boschelli, D. H., Ye, F., Golas, J. M., & Boschelli, F. (2004). Synthesis and inhibition of Src kinase activity by 7-ethenyl and 7-ethynyl-4-anilino-3-quinolinecarbonitriles. Bioorganic & Medicinal Chemistry Letters (Vol. 14). Crombie, A. L., Sum, F. - W., Powell, D. W., Hopper, D. W., Torres, N., Berger, D. M., et al. (2010). Synthesis and biological evaluation of tricyclic anilinopyrimidines as IKKbeta inhibitors. Bioorganic & Medicinal Chemistry Letters. Emmitte, K. A., Wilson, B. J., Baum, E. W., Emerson, H. K., Kuntz, K. W., Nailor, K. E., et al. (2009). Discovery and optimization of imidazo[1,2-a]pyridine inhibitors of insulin-like growth factor-1 receptor (IGF-1R). Bioorganic & Medicinal Chemistry Letters (Vol. 19). Freiberg, G., Wilkins, J., David, C., Kofron, J., Jia, Y., Hirst, G. C., et al. (2004). Utilization of microarrayed compound screening (microARCS) to identify inhibitors of p56lck tyrosine kinase. J Biomol Screen (Vol. 9). Jin, G., Aulabaugh, A., Pocas, J., Liu, H., Kriz, R., & Sampath, D. (2006). Development and comparison of nonradioactive in vitro kinase assays for NIMA-related kinase 2. Anal Biochem (Vol. 358). Kuroda, K., Kobayashi, K., Kitagawa, Y., Nakagawa, T., Tsumura, H., Komeda, T., et al. (2008). Efficient Antibody Production upon Suppression of O Mannosylation in the Yeast Ogataea minuta. Appl Environ Micro (Vol. 74). Li, M., Luraghi, P., Amour, A., Qian, X. - D., Carter, P. S., Clark, C. J., et al. (2009). Kinetic assay for characterization of spleen tyrosine kinase activity and inhibition with recombinant kinase and crude cell lysates, Anal Biochem (Vol. 384). Lindqvist, L., Oberer, M., Reibarkh, M., Cencic, R., Bordeleau, M. - E., Vogt, E., et al. (2008). Selective Pharmacological Targeting of a DEAD Box RNA Helicase. PLoS ONE (Vol. 3). Moshinsky, D. J., Ruslim, L., Blake, R. A., & Tang, F. (2003). A Widely Applicable, High-Throughput TR-FRET Assay for the Measurement of Kinase Autophosphorylation: VEGFR-2 as a Prototype. J Biomol Screen (Vol. 8). Patnaik, D., Jun Xian,, Glicksman, M. A., Cuny, G. D., Stein, R. L., & Higgins, J. M. G. (2008). Identification of Small Molecule Inhibitors of the Mitotic Kinase Haspin by High-Throughput Screening Using a Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer Assay. J Biomol Screen (Vol. 13). Sabbatini, P., Rowand, J. L., Groy, A., Korenchuk, S., Liu, Q., Atkins, C., et al. (2009). Antitumor Activity of GSK1904529A, a Small-molecule Inhibitor of the Insulin-like Growth Factor-I Receptor Tyrosine Kinase. Clin Cancer Res (Vol. 15). Sadler, T. M., Achilleos, M., Ragunathan, S., Pitkin, A., LaRocque, J., Morin, J., et al. (2004). Development and comparison of two nonradioactive kinase assays for I kappa B kinase. Anal Biochem (Vol. 326). Schlapbach, A., Feifel, R., Hawtin, S., Heng, R., Koch, G., Moebitz, H., et al. (2008). Pyrrolo-pyrimidones: A novel class of MK2 inhibitors with potent cellular activity. Bioorganic & Medicinal Chemistry Letters (Vol. 18). Vassilev, L. T., Tovar, C., Chen, S., Knezevic, D., Zhao, X., Sun, H., et al. (2006). Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. PNAS (Vol. 103). Velcicky, J., Feifel, R., Hawtin, S., Heng, R., Huppertz, C., Koch, G., et al. (2010). Novel 3-aminopyrazole inhibitors of MK-2 discovered by scaffold hopping strategy. Bioorganic & Medicinal Chemistry Letters (Vol. 20). Wang, Y., Shakespeare, W. C., Huang, W. - S., Sundaramoorthi, R., Lentini, S., Das, S., et al. (2008). Novel N9-arenethenyl purines as potent dual Src/Abl tyrosine kinase inhibitors. Bioorganic & Medicinal Chemistry Letters (Vol. 18). Wei, M., Wynn, R., Hollis, G., Liao, B., Margulis, A., Reid, B. G., et al. (2007). High-Throughput Determination of Mode of Inhibition in Lead Identification and Optimization. J Biomol Screen (Vol. 12). Zhang, W. - G., Shor, B., & Yu, K. (2006). Identification and characterization of a constitutively T-loop phosphorylated and active recombinant S6K1: Expression, purification, and enzymatic studies in a high capacity non-radioactive TR-FRET Lance assay. Protein Expression and Purification (Vol. 46). Zhang, W. X., Wang, R., Wisniewski, D., Marcy, A. I., LoGrasso, P., Lisnock, J. - M., et al. (2005). Time-resolved Forster resonance energy transfer assays for the binding of nucleotide and protein substrates to p38alpha protein kinase. Anal Biochem (Vol. 343).
ECLASS 10.1	42051003
ECLASS 11.0	42051003
UNSPSC	12000000
Available
Manufacturer - Applications	Flow Cytometry/FACS\|HTS\|TR-FRET
Manufacturer - Targets	Biotin
Description	Streptavidin conjugated toSureLight Allophycocyanin for kinase assays, 1mg
Immunogen	NA
Storage Buffer	10 mM tris (pH 8.2), 150 mM NaCl, 1 ?g/mL pentachlorophenol, 0.1% BSA and 50mM Sucrose
Excitation max	652
Emission max	657.5
Form / Storage	Lyophilized, Store at 4° C.
Stability	Upon reconstitution, product should be stored at 4° C and used within 8 months.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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COL-D3-2212-K01-1mg-datasheet.pdf

COL-D3-2212-K01-1mg-safety-datasheet.pdf