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Cyclic AMP TR-FRET kit, 1000 tests

Cyclic AMP TR-FRET kit, 1000 tests

Item no.	COL-AD-cAMP-1K
Manufacturer	Columbia Biosciences
Amount	1,000 tests
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Manufacturer - Applications	TR-FRET
Manufacturer - Conjugate / Tag	Europium
Description	This kit is designed to detect and quantitatively measure the amount of cyclic AMP (cAMP) present in cell-based assay systems. A europium (EU) labeled monoclonal anti-cAMP antibody (donor) is paired with a fluorescently labeled cAMP analog (Acceptor), and in the absence of any endogenous cAMP, will produce a time-resolved energy transfer signal at 665 nm when excited at 340 nm. If cAMP accumulates in the well due to the activation of cellular adenylyl cyclase, this cAMP will compete with the acceptor and will inhibit the formation of the labeled Donor/Acceptor complex thereby decreasing the amount of the energy transfer signal produced. The overall drop in signal is inversely proportional to the amount of cAMP accumulated.
Excitation max	340
Emission max	665
Technical Info	Advantages: homogeneous assay, just mix and read no wash steps-TR-FRET format increases sensitivity eliminates compound interference allows for HTS assays to be performed in a miniaturized format

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.