Comparison

SureLight™ PE-665 (Cyanine 5.0)

Item no. COL-D14-000-5mg
Manufacturer Columbia Biosciences
Amount 5 mg
Category
Type Fluorescent dyes and probes
Format Liquid
Applications WB, FC, ELISA
Specific against other
Conjugate/Tag PE
ECLASS 10.1 30196090
ECLASS 11.0 30196090
UNSPSC 12000000
Available
Manufacturer - Conjugate / Tag
SureLight PE-665
Description
Many applications and instruments were developed specifically for PE-665 (PE Cyanine 5.0). It is commonly used in immunoassays such as FACS, flow cytometry, multimer/tetramer applications. With new instrumentation available, PE-665 is also well suited for immunohistochemistry (IHC) and Western blot applications.
SureLight™ PE-665 (PE Cyanine 5.0) Labeling Protocol:
Click here to be redirected to a well-documented conjugation protocol - http://www.drmr.com/abcon/C5P.html
REF: Hardy, RR: Purification and coupling of fluorescent proteins for use in flow cytometry. In: Handbook of Experimental Immunology, 4th ed. DM Weir, LA Herzenberg, C Blackwell, and LA Herzenberg, editors. Blackwell Scientific Publications, Boston, 1986, pp. 31.1-31.12.
Excitation max
488nm
Emission max
665nm
Form / Storage
Supplied as liquid. Upon receipt, store at 2-8C. DO NOT FREEZE.
Stability
Product should be stored at 2–8C in the dark.
Technical Info
Structural Characteristics
R-phycoerythrin is predominantly produced by red algae. The protein is made up of at least three different subunits and varies according to the species of algae that produces it. The subunit structure of the most common R-PE is (αβ)6γ. The α subunit has two phycoerythrobilins (PEB), the β subunit has 2 or 3 PEBs and one phycourobilin (PUB), while the different gamma subunits are reported to have 3 PEB and 2 PUB (γ1) or 1 or 2 PEB and 1 PUB (γ2). The molecular weight of R-PE is 250, 000 Daltons. SureLight™ PE-665 is a tandem fluorophore comprising R-Phycoerythin and a red-shifted cyanine dye.
Absorption maximum: 565 nm
Additional Absorption peak: 498 nm

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 mg
Available: In stock
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