Comparison

Anti TDP-43, phospho Ser409/410

Item no. CAC-TIP-PTD-M01
Manufacturer Cosmobio
Amount 100 ul
Category
Type Antibody Monoclonal
Format Supernatant
Applications WB, IHC, ELISA
Clone 11-9
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Similar products TDP-43, phospho Ser409/410
Available
Storage Conditions
below -20°C and below -70°C for prolonged storage; aliquot to avoid freeze/thaw cycles.
Description
Anti-TAR DNA-Binding Protein 43 (TDP-43), phospho-Ser409/410 mAb 11-9 was cloned from mice immunized with diphospho-peptide [CMDSKS(p)S(p)GWGM]. 11-9 recognizes human TDP-43 phosphorylated on serines 409 and 410. 11-9 is validated for western blot, ELISA and IHC(f). This antibody is a powerful tool for biochemical and immunohistochemical analyses of TDP-43 proteinopathy.

TDP-43, a heterogeneous nuclear ribonucleoprotein, was identified as a component of ubiquitin-positive and tau-negative inclusions observed in cases of frontotemporal lobar degeneration (FTLD-U) and amyotrophic lateral sclerosis (ALS). Immunochemical analyses using antibodies generated against phospho- and non-phosphopeptides of human TDP-43 revealed that abnormally phosphorylated full-length TDP-43 (45 kDa), C-terminal fragments (~25 kDa) and smearing substances are deposited as intracellular inclusions in affected regions of FTLD-U and ALS cases.

This antibody is a powerful tool for biochemical and immunohistochemical analyses of neurodegenerative diseases and for evaluation of cellular or animal models of TDP-43 proteinopathy.

Specifications
Important Notice About Format Change:
Replacement for Cat No.: CAC-TIP-PTD-M01
For lots "11-9-20" and newer, this antibody is now provided as ammonium sulfate precipitated culture supernate, formulated in 50% glycerol, 0.05% NaN3. Older lots were provided as raw culture supernate. Vials of the previous format remain available while supplies last. (posted: March 2020). This has improved sensitivity, and does not require altering current protocols.

Recommended dilutions:

  • Western blotting:1/1000-1/3000
  • Immunohistochemistry: 1/3000-1/10000
  • ELISA: 1/1000-1/5000
  • Other applications not tested
  • Optimal dilutions/concentrations should be determined by end user.

References

    1. Inukai Y, Nonaka T, Arai T, et al. Abnormal phosphorylation of Ser409/410 of TDP-43 in FTLD-U and ALS. FEBS Lett. 582, 2899-2904, 2008 PubMed: 18656473
Purification
affinity purified
Immunogen
CMDSKS(p)S(p)GWGM, S(p): phosphoserine 409/410
Manufacturer - Specificity
phospho-TDP-43

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.