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Anti Collagen 4 (ALPHA)5(IV) mAb (Clone B51)

Anti Collagen 4 (ALPHA)5(IV) mAb (Clone B51)

Item no.	SGE-C451
Manufacturer	Cosmobio
Amount	500 ul
Category	Antibodies Cell Biology Western Blot Cell Stainings ECM Primary Antibodies
Type	Antibody Monoclonal
Applications	IF
Clone	B51
Specific against	Human (Homo sapiens)
Host	Rat
Isotype	IgG2a
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Available
Manufacturer - Category	Antibodies/Primary Antibodies/Cell Biology/Cytoskeleton; Antibodies/Primary Antibodies/Cell Biology/Extracellular Matrix; Antibodies/Primary Antibodies/Cell Biology/Signal Transduction; Antibodies/Primary Antibodies/Disease/Alport Syndrome; Antibodies/Primary Antibodies/Disease/Goodpastures Syndrome; Antibodies/Primary Antibodies/Organs & Organ Systems/Kidney; Research Area/Cell Biology/Cytoskeleton/Primary Antibodies; Research Area/Cell Biology/Extracellular Matrix/Primary Antibodies; Research Area/Cell Biology/Signal Transduction/Primary Antibodies; Research Area/Disease/Alport Syndrome/Primary Antibodies; Research Area/Disease/Goodpastures Syndrome/Primary Antibodies; Research Area/Organs & Organ Systems/Kidney/Primary Antibodies
Maker	SGE
Antigen/Source	Collagen 4 alpha5(IV) chain
Description	Check out our Human Collagen IVα Rat Monoclonal Antibodies Dashboard for more information about this product and additional epitope-defined monoclonal antibodies useful for research on hereditary diseases related to collagen IV, including our popular Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25) (SGE-CFT45325). Alport syndrome is an inherited disease characterized by the pathological absence or reduction of the collagen α 5(IV) chain in glomerular basement membrane (GBM), tubular basement membrane (TBM) and Bowman's capsular basement membrane. Anti Collagen 4 (ALPHA)5(IV) mAb (Clone B51) (cat no. SGE-C451) is an unlabeled version of one of three different rat mAbs included in Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25) (SGE-CFT45325) for easy and rapid staining of human renal and skin biopsy sections to distinguish Alport syndrome from normal tissue. Anti-Collagen 4 Cocktail mAb (Clones H53, B51, H25) comprises two different FITC-conjugated mAbs (clones H53 and B51) that reveal the Alport-affected α 5(IV) chain in glomerular, tubular and Bowman's capsular BMs and an internal positive control Texas Red-conjugated mAb (clone H25) that targets α 2(IV) to reveal endothelial basement membrane structure Product specifications	Specification	Detail Anti Collagen 4 (ALPHA)5(IV) mAb (Clone B51) Cat No \| SGE-C451 Application \| Staining of human cryostat sections by indirect immunofluorescence (acid-urea treatment not required) Quantity \| 0.5 ml Appearance \| Solution Purification \| Diluted ascitic solution obtained from nude mice; diluted with culture medium containing 10% fetal bovine serum and 0.1% NaN3 Fluorescent labeling \| none Clone name \| B51 (rat IgG2a) Specificity \| B51 is specific to the NC1 domain of human α5(IV). Not reactive with human α 1, α 2, α 3, α 4 and α 6). (Reference 1). Antibodies preparation \| Monoclonal antibody was prepared by the rat lymph node method developed by Shigei Medical Research Institute with a synthetic peptide of type IV collagen as immunogen. Storage \| In the dark at 2-4° C or below -20° C. Stable for several years under these conditions. Source \| Shigei Medical Research Institute, 2117 Yamada, Okayama 701-0202, Japan; TEL: +81-86-282-3113; FAX: +81-86-282-3115; E-mail: inst@shigei.or.jp References Kagawa M et al. Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport syndrome. Nephrol. Dial. Transplant. 12: 1238-1241 (1997) (PMID: 9198058) Borza DB et al. The NCI domain of collagen IV encodes a novel network composed of the α 1, α 2, α 5, and α 6 chains in smooth muscle basement membranes. J. Biol. Chem. 276: 28532-28540 (2001) (PMID: 11375996) Sado et al. Establishment by the rat lymph node method of epitope-defined monoclonal antibodies recognizing the six different α chains of human type IV collagen. Histochem. Cell Biol. 104: 267-275 (1995) (PMID: 8548560) - Yoshioka K et al. Type IV collagen α 5 chain: Normal distribution and abnormalities in X-linked Alport syndrome revealed by monoclonal antibody. Am. J. Pathol. 144: 986-996 (1994) (PMID: 8178947) - Ninomiya Y et al. Differential expression of two basement membrane collagen genes, COL4A6 and COL4A5, demonstrated by immunofluorescence staining using peptide-specific monoclonal antibodies. J. Cell Biol. 130: 1219-1229 (1995) (PMID: 7657706) - Naito I et al. Relationship between COL4A5 gene mutation and distribution of type IV collagen in male X-linked Alport syndrome. Kidney Int. 50: 304-311 (1996) (PMID: 8807602) Citations - Bu L et al. Somatic Mosaicism in a Male Patient With X-linked Alport Syndrome. Kidney Int Rep. 14(4): 1031-1035 (2019) (PMID: 31312776) - Samar M et al. Negative Staining for COL4A5 Correlates With Worse Prognosis and More Severe Ultrastructural Alterations in Males With Alport Syndrome. Kidney Int Rep. 2(1): 44-52 (2017) (PMID: 29142939) - Malone AF et al. Functional assessment of a novel COL4A5 splice region variant and immunostaining of plucked hair follicles as an alternative method of diagnosis in X-linked Alport syndrome. Pediatr Nephrol. 32(6): 997-1003 (2017) (PMID: 28013382) - Nozu K et al. X-linked Alport syndrome caused by splicing mutations in COL4A5. Am Soc Nephrol. 9(11): 1958-64 (2014) (PMID: 25183659) - Matsubara S et al. Pregnancy complicated with Alport syndrome: a good obstetric outcome and failure to diagnose an infant born to a mother with Alport syndrome by umbilical cord immunofluorescence staining. Obstet Gynaecol Res. 35(6): 1109-14 (2009) (PMID: 20144175) - Patey-Mariaud de Serre N et al. Collagen alpha5 and alpha2(IV) chain coexpression: analysis of skin biopsies of Alport patients. Kidney Int. 72(4): 512-6 (2007) (PMID: 17554254) - Kharrat M et al. Autosomal dominant Alport's syndrome: study of a large Tunisian family. Kidney Dis Transpl. 17(3): 320-5 (2006) (PMID: 16970251)
Clonality	Monoclonal
Immunogen	NC1 domain of alpha5(IV)
Fraction	Supernatant

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 500 ul

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Delivery expected until 11/13/2025

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