Westase Yeast Cell Lytic Enzymes

Biomolecules/Proteins/Fungus; Research Area/Immunology & Inflammation/Fungus/Proteins; Sample Preparation/Cell & Tissue Disruption/Enzymes; Instruments & Labware/Cell & Tissue Disruption/Enzymes; Nucleic Acid Engineering/Cell & Tissue Disruption/Enzymes; Protein Engineering/Cell & Tissue Disruption/Enzymes; Cells & Cell Culture/Cell & Tissue Disruption/Enzymes; Biomolecules/Fungus/Proteins

Application:
Digestive enzyme of cell walls of yeast Westase was prepared from liquid culture supernatant of Streptomyces rochei DB-34. This product has complex lytic activities of yeast cell mainly consisting of (beta)-1, 6 glucanase and (beta)-1, 3 glucanase activity. This enzyme works well forAscosporogenous yeasts such as Saccharomyces cerevisiae. But it is also available for fission yeasts such as Schizosaccharomyces pombe which cannot be protoplasted just with Zymolyase treatment or Basidiosporogenous yeasts, Imperfect yeasts such as Ustilago maydis, Phaffia rhodozyma, Cryptococcus albidus, etc. which are rarely protoplasted, and make them efficiently into protoplast.
Definition of Activity: (beta)-1, 6 glucanase activity
One unit is defined as the amount required to release 1 (mu)mol reducing sugar from 10 mg/ml Pustulan solution in 1 min. at 37° C, pH6.0.
Lytic activity: One unit is defined as the amount required to cause a 1% decrease in absorbance at 660 nm in 1 min. at 30° C, pH6.0 when using cell wall fraction of Cryptococcus albidus IFO 0612
Specific activities: (beta)-1, 6 glucanase activity : > 400 units / g
Lytic activity : > 35, 000 units / g
DNase activity : ND (McIlvain Buffer, pH6.0)
Storage: Lyophilized preparation is stable for at least 1 year when stored at 4° C.