MagExtractor - PCR & Gel Clean up

MagExtractor-PCR & Gel Clean Up is a magnetic silica bead-based kit for rapid, simple and reliable purification of DNA fragments from PCR reactions and gel extractions. Requiring a suitable magnetic separation stand like Magical Trapper (TYB-MGS-101), it exploits the property of silica surfaces to bind DNA in the presence of chaotropic agents (reference 1). Purified DNA can be used directly in a multitude of downstream applications.

Applications

• PCR fragment isolation
  


• DNA isolation from agarose gel blocks
  


• Desalination
  


• dNTP removal
  


• Primer removal
  


• Enzyme removal
  

Purified DNA can be used directly in:

• PCR reactions
  


• Sequencing reactions
  


• Labeling reactions
  


• Ligation reactions
  


• Enzymatic reactions
  


• Genetic transformation
  


• Hybridization
  

Features

• Recoverable DNA fragment size ranges from ~100 to 50, 000bp. Fragments < 40bp are mostly excluded.
  


• Maximum binding capacity is ~5ug DNA/30ul beads. However, we recommend using 2.5ug/30ul beads as a practical guideline.
  


• DNA purification is possible directly from various reaction solutions including but not limited to those for PCR, alkaline phosphatase and other enzymes.
  


• Recovery yield for ~100bp-50kbp-sized DNA fragments from ~100ul of reaction solution is 60-70%.
  


• Processing time for DNA fragment recovery is ~5 minutes from DNA solutions and < 15 minutes from agarose gel blocks.
  


• Because agarose gel slices dissolve in the Binding Solution at room temperature, heat blocks are not required.
  

Kit contents (sufficient for 200 purifications)

• Binding Solution 88 ml (store at RT shielded from light)
  


• Magnetic Beads 8.5 ml (store at RT)
  


• Washing Solution 132 ml (store at RT)
  

Note: Exposure of the Binding or Washing solutions to low temperature may result in crystal precipitation. In this case, before use dissolve crystals by manual gentle end-over-end mixing. Also, as Binding and Washing solutions contain protein denaturant, exercise caution when handling and immediately rinse and clean off any solution that may get on the body.

References

1. B. Vogelstein and D. Gillespie, Proc. Natl. Acad. Sci. USA. 76: 615-619 (1979)