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Control for Ubiquitin Affinity Beads

Control for Ubiquitin Affinity Beads

Item no.	CUB02-beads
Manufacturer	Cytoskeleton
Amount	10 assays
Category	Cell Biology Cell Status Autophagy Reagents & Chemicals Nanoparticles Beads & Resins
Type	Antibody Conjugated Beads
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	41105507
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Associated Products	Signal-Seeker Ubiquitination Detection Kit (Cat. # BK161) Signal-Seeker Ubiquitin Affinity Beads (Cat.# UBA01-beads) Signal-Seeker: BlastR Rapid Lysate Prep Kit (Cat. # BLR01) Signal-Seeker: PTMtrue Ubiquitin Antibody (Cat.# AUB01)
Description	As part of the Signal-Seeker product line, ubiquitination control beads (CUB02) have been developed as highly specific negative control beads for Cytoskeleton's ubiquitination affinity bead (UBA01-Beads) products. These control beads are far superior to beads alone, because it has mutated versions of the UBDs covalently attached to the control bead matrix. This control is particularly important for ubiquitin IPs as the unmodified version of target proteins often bind non-specifically to all available ubiquitin enrichment affinity matrices. A comprehensive Signal-Seeker Ubiquitin Detection Kit (BK161), which includes ubiquitin control beads (CUB02), is available and is recommended for first time users.
Manufacturer - Amount	Each package contains enough ubiquitin control beads for 10 reactions.
Validation Data	Ubiquitination Control Beads White Paper Each lot of affinity-bead is quality controlled to provide high batch to batch consistency, see COA documents. Application 1: Utilization of IgG control beads
Figure 1 Legend	Figure 1: Swiss 3T3 cells were pre-treated with MG-132, then either untreated or treated with CNF1 (CN04) for 3 hours prior to lysis with BlastR buffer. The BK161 kit was utilized to perform the IP on 300 ?g of lysate per condition. Input: 3 ?g input untreated lysate, Ubiquitin Affinity beads (UBA01) plus untreated lysate, UBA01 plus CN04 treated lysate, ubiquitin control beads (CUB02) plus untreated lysate, and CUB02 plus CN04 treated lysate. Samples were analyzed for Rac1 ubiquitination using an anti-Rac1 antibody.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

CUB02-beads.pdf

CUB02-beads-datasheet.pdf

CUB02-beads-safety-datasheet.pdf