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Rap1b protein (Human wild type, His)

Rap1b protein (Human wild type, His)

Item no.	RR02-A
Manufacturer	Cytoskeleton
Amount	1 x 100 ug
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton small GTPases Ras Subfamily Rho Subfamily Rap Subfamily
Type	Proteins
Specific against	Human (Homo sapiens)
Purity	Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Rap1b samples are >90% pure (Figure 1).
Citations	Coming soon! For the most recent publications citing this product, please contact our Technical Service department at infohoelzel.de
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, Rap1b, Rap 1b, Rap1b protein, human Rap1b
Similar products	Rap1b, Small G protein, Rap 1b, Rap1b protein, human Rap1b
Shipping Condition	Room temperature
Available
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	7
Product Uses	Rap1b biochemistry Rap1b GTPase assays Rap1b nucleotide exchange assays Rap1b binding studies
Material	The human Rap1b protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl₂ 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02. The recombinant protein is approximately 26 kDa, consisting of the Rap1b protein plus a linker and a 6X His tag in the amino-terminus. For many other purified small G-proteins, see our main small G-protein product page.
Biological Activity	The biological activity of Rap1b is determined from its ability to exchange GDP for GTP. EDTA is used to sequester magensium ions from the Rap1b protein, thereby stimulating nucleotide exchange activity. The RhoGEF exchange assay Biochem Kit (Cat. # BK100) is used to monitor the exchange (Figure 2).
Figure 1 Legend	Rap1b His-tagged protein purity determination. A 10 ug sample of RR02 (molecular weight approx. 26 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.
Figure 2 Legend	Rap1b GTP exchange assay using BK100. Rap1b protein (1 uM) was mixed with exchange buffer and aliquoted to four wells of a 96-well half area plate. After 5 cycles of reading in a fluorimeter, EDTA was added to 40 mM in the test samples and Milli-Q water to the control samples. Reactions were monitored for 30 min.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

RR02-A.pdf

RR02-A-datasheet.pdf

RR02-A-safety-datasheet.pdf