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Bovine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

Bovine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

Manufacturer	DlDevelop
Category	Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay Neuronal Cell Markers
Type	Elisa-Kit
Specific against	Bovine
Amount	96T
Item no.	DL-BDNF-b-96T
Targets	BDNF;;NTF3
eClass 6.1	32160605
eClass 9.0	32160605
Available
Short description	For quantitatively determining the concentration in the customer's samples
Sensitivity	0.16ng/mL
Detection range	0.625-40ng/mL
Antigen	BDNF
Certified	CE&ISO
ISO- certification number	17916Q10078ROS
Cross Reaction	This assay has high sensitivity and excellent specificity for detection of this index. No significant cross-reactivity or interference between this index and analogues was observed.
Intended Use	This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Kit Content	Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 2 Standard 2 Standard Diluent 1×20mL Detection Reagent A 1×120?L Assay Diluent A 1×12mL Detection Reagent B 1×120?L Assay Diluent B 1×12mL TMB Substrate 1×9mL Stop Solution 1×6mL Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Principle of the Elisa	The microtiter plate provided in this kit has been pre-coated with an antibody specific to this index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to this index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain this index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of this index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Storage and Stability	1.For unopened kit: All the reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer (30 × concentrate) and the Stop Solution should be stored at 4oC upon receipt while the others should be at -20oC. 2.For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Reproducibility	The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Experimental method	Sandwich
Shelf life	12 months
Gene ID	617701
UniProtKB	Q95106

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96T

available

Delivery expected until 5/23/2024