Mouse NN-T4(Neonatal Thyroxine) ELISA Kit

2-8°C

Colormetric

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse NN-T4. During the reaction, Mouse NN-T4 in the sample or standard competes with a fixed amount of Mouse NN-T4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse NN-T4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase(HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse NN-T4 in the samples is then determined by comparing the OD of the samples to the standard curve.