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ProAKAP4 ELISA Kit (Rodent and Human) European Partner

Item no. 4BDX-18K12RUO
Manufacturer 4BioDx
Amount 96 wells
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Horse (Equine), Bull, Ram
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Designation
ProAKAP4 ELISA Kit (Rodent and Human)
Shipping conditions
Room temperature
Storage temperature at reception
+4°C
Shelf life
12 months
Short Description
The ProAKAP4 ELISA Kit is the first ELISA assay that objectively validates semen quality by quantifying a protein biomarker called proAKAP4 in sperm samples (in ejaculate, chilled or frozen semen in extender or in isolated spermatozoa).
Design Principle
Sandwich-based
Result Output
Quantitative
Method of Detection
Colorimetric
Compatible Sample Types
Ejaculates, semen in extenders, Isolated spermatozoa)
Run Time
2h to 3h
Number of Samples Analyzed per Kit
From one to 88 samples can be run simultaneously or individually (12 breakable strips of 8 wells per 96 well microplate / per kit)
Use
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Sensitivity
4 ng / mL
Detection Range
0 to 150 ng
References
Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86.
Delehedde M, Carracedo S, Selleslagh M, et al. ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. (2019); 2(1):13-19
Escalier D and Albert M (2006) New fibrous sheath anomaly in spermatozoa of men with consanguinity. Fertility and Sterility. Vol. 86:1-9.
Jumeau F, Sigala J, Dossou-Gbete F, Frimat K, Barbotin AL, Buée L, Béhal H, Sergeant N and Mitchell V (2018) A-kinase anchor protein 4 precursors (pro-AKAP4) in human sperm. Andrology. Vol. (6):854-859.
Jumeau F, Sigala J, Fernandez-Gomez FJ, Eddarkaoui S, Duban-Deweer S, Buée L, Béhal H, Sergeant N and Mitchell V (2018) Gel electrophoresis of human sperm: a simple method for evaluating sperm protein quality. Basic Clinical Andrology. Vol. (28)10:1-8.
Luconi M, Cantini G, Baldi E and Forti G (2011) Role of A-kinase anchoring proteins (AKAPs) in reproduction. Frontiers in Bioscience. Vol. 16:1315-1330.
Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19.
Brown PR, Miki K, Harper D and Eddy EM (2003) A-kinase anchoring protein 4 binding proteins in the fibrous sheath. Biology of Reproduction. Vol. 68:2241-2248.
Miki K, Goulding EH, Fulcher KD, Eddy E, Willis WD and Brown PR (2002) Targeted disruption of the AKAP4 gene causes defects in sperm flagellum and motility. Developmental Biology. Vol. 248:331-342.
Kit Components
R1 - Capture Antibody Coated Microplate of 96-wells (12 x 8-well strips) R2 - 1 Bottle of 10x Washing Buffer Solution (30 mL)
R3 - 1 Bottle of 1x Dilution Buffer (30 mL)
R4 - 7 Vials of Ready-to-Use Standard Solution (0.7 mL per vial)
R5 - 1 Bottle of 1x Bull Spermatozoa Lysis Buffer (30 mL) R6 - 1 Vial of Detection Antibody (0.1 mL)
R7 - 1 Bottle of Substrate Solution (11 mL)
R8 - 1 Bottle 1x Stop Solution (6 mL)
R9 - 1 Vial of Positive Control (0.7 mL) R10 - 2 Adhesive Plate Sealers
R11 - User Manual Instructions
Other Materials Required
- 96-well microplate reader measuring absorbance at 450 nm
- Horizontal orbital microplate shaker (300 rpm)
- Vortex
- Multichannel pipette of 300 μL
- Pipettes of 20 μL, 200 μL and 1000 μL
- Pipette tips
- Polypropylene microtubes of 1.5 mL (for sample preparation)
- One polypropylene tube of 15 mL (for R6 dilution)
- Plastic reagent reservoirs
- One glass bottle (for R2 dilution)
- Scissors
- Pen
- Aluminum foil
- Ultrapure or double deionized water
Protocol Summary
Prepare solution and samples as instructed in the User Manual.
Always read the User Manual before use carefully

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 wells
Available: In stock
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