Cat 4MID kit (ELISA kit)

Cat 4MID kit (ELISA kit)

+4°C

The Cat 4MID® KIT is the first ELISA assay that objectively validates semen quality by quantifying a protein biomarker called proAKAP4 in sperm samples (in ejaculate, chilled or frozen semen in extender or in isolated spermatozoa).

Quantitative

Ejaculates, semen in extenders, Isolated spermatozoa)

From one to 88 samples can be run simultaneously or individually (12 breakable strips of 8 wells per 96 well microplate / per kit)

4 ng / mL

Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448 - P88 https://www.sciencedirect.com/science/article/abs/pii/S0378432020303201
Huffmeyer AA, Pukazhenthi BS and Wayne RK (2021) Differential gene expression patterns in spermatozoa from teratospermic and normospermic domestic cats. Anim Reprod Sci. Vol. 226:106698. doi: 10.1016/j.anireprosci.2021.106698.
Griffin R, Swegen A, Baker M, John Aitken R, A Skerrett-Byrne D, Silva Rodriguez A, E Martín-Cano F, Nixon B, J Peña F, Delehedde M, Sergeant N and Gibb Z (2020) Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction. 160(5):695-707.
Delehedde M, Carracedo S, Selleslagh M, Eddarkaoui S, Amirat-Briand L and Sergeant N (2019) ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. Vol. 2(1):13-19.
Blommaert D, Sergeant N, Delehedde M, Jouy N, Mitchell V, Franck T, Donnay I, Lejeune JP and Serteyn D (2019b) Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. Vol. 131:52-60.
Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19.

- 96-well microplate reader measuring absorbance at 450 nm
- Horizontal orbital microplate shaker (300 rpm)
- Vortex
- Multichannel pipette of 300 μL
- Pipettes of 20 μL, 200 μL and 1000 μL
- Pipette tips
- Polypropylene microtubes of 1.5 mL (for sample preparation)
- One polypropylene tube of 15 mL (for R6 dilution)
- Plastic reagent reservoirs
- One glass bottle (for R2 dilution)
- Scissors
- Pen
- Aluminum foil
- Ultrapure or double deionized water