Dog Thyroid Stimulating Hormone (TSH) ELISA Kit (CLIA)

Manufacturer Biomatik
Type Elisa-Kit
Specific against Canine
Applications CLIA
Amount 96T
Item no. BM-EKU11558
eClass 6.1 32160605
eClass 9.0 32160605
96-Well Plate
Target Name
Thyroid Stimulating Hormone
Target Synonyms
Sample type
Serum, plasma and other biological fluids
Detection ra nge
78.1-20, 000 pg/mL
29.4 pg/mL
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Stimulating Hormone (TSH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%.
Detection Method
Assay Time
2 hours
Assay Type
Competitive Inhibition
Shipping Condition
Ice packs
Short term: 4CC; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date
8 months
UniProt ID
This assay has high sensitivity and excellent specificity for detection of Thyroid Stimulating Hormone (TSH).
Cross Activity
This assay has high sensitivity and excellent specificity for detection of Thyroid Stimulating Hormone (TSH).
Test principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Thyroid Stimulating Hormone (TSH). A competitive inhibition reaction is launched between biotin labeled Thyroid Stimulating Hormone (TSH) and unlabeled Thyroid Stimulating Hormone (TSH) (Standards or samples) with the pre-coated antibody specific to Thyroid Stimulating Hormone (TSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thyroid Stimulating Hormone (TSH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Thyroid Stimulating Hormone (TSH) level in the sample or standard.
Assay procedure summary
1. Prepare all reagents, samples and standards; 2. Add 50 uL standard or sample to each well.         And then add 50 uL prepared Detection Reagent A immediately.         Shake and mix. Incubate 1 hour at 37CC; 3. Aspirate and wash 3 times; 4. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37CC; 5. Aspirate and wash 5 times; 6. Add 100 uL Substrate Solution. Incubate 10 minutes at 37CC; 7. Read RLU value immediately.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance
Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96T
Available: In stock

Delivery expected until 6/27/2024 


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