Human Neuropeptide FF (NPFF) ELISA Kit (CLIA)

Manufacturer Biomatik
Type Elisa-Kit
Specific against Human
Applications CLIA
Amount 96T
Item no. BM-EKU11563
eClass 6.1 32160605
eClass 9.0 32160605
96-Well Plate
Target Name
Neuropeptide FF
Target Synonyms
FMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection ra nge
12.35-1, 000 pg/mL
4.99 pg/mL
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide FF (NPFF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide FF (NPFF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%.
Detection Method
Assay Time
2 hours
Assay Type
Competitive Inhibition
Shipping Condition
Ice packs
Short term: 4CC; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date
8 months
UniProt ID
This assay has high sensitivity and excellent specificity for detection of Neuropeptide FF (NPFF).
Cross Activity
This assay has high sensitivity and excellent specificity for detection of Neuropeptide FF (NPFF).
Test principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Neuropeptide FF (NPFF). A competitive inhibition reaction is launched between biotin labeled Neuropeptide FF (NPFF) and unlabeled Neuropeptide FF (NPFF) (Standards or samples) with the pre-coated antibody specific to Neuropeptide FF (NPFF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuropeptide FF (NPFF) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Neuropeptide FF (NPFF) level in the sample or standard.
Assay procedure summary
1. Prepare all reagents, samples and standards; 2. Add 50 uL standard or sample to each well.         And then add 50 uL prepared Detection Reagent A immediately.         Shake and mix. Incubate 1 hour at 37CC; 3. Aspirate and wash 3 times; 4. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37CC; 5. Aspirate and wash 5 times; 6. Add 100 uL Substrate Solution. Incubate 10 minutes at 37CC; 7. Read RLU value immediately.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance
Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96T
Available: In stock

Delivery expected until 6/27/2024 


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