Human Coagulation Factor VIII (F8) ELISA Kit (CLIA)

Manufacturer Biomatik
Type Elisa-Kit
Specific against Human
Applications CLIA
Amount 96T
Item no. BM-EKU11644
eClass 6.1 32160605
eClass 9.0 32160605
96-Well Plate
Target Name
Coagulation Factor VIII
Target Synonyms
FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein
Sample type
Detection ra nge
13.7-10, 000 pg/mL
4.9 pg/mL
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor VIII (F8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor VIII (F8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%.
Detection Method
Assay Time
2 hours, 40min
Assay Type
Double-antibody Sandwich
Shipping Condition
Ice packs
Short term: 4CC; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date
8 months
UniProt ID
This assay has high sensitivity and excellent specificity for detection of Coagulation Factor VIII (F8).
Cross Activity
This assay has high sensitivity and excellent specificity for detection of Coagulation Factor VIII (F8).
Test principle
The microplate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VIII (F8). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Coagulation Factor VIII (F8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Coagulation Factor VIII (F8) level in the sample or standard.;
Assay procedure summary
1. Prepare all reagents, samples and standards; 2. Add 100 uL standard or sample to each well. Incubate 2 hours at 37CC; 3. Aspirate and add 100 uL prepared Detection Reagent A. Incubate 1 hour at 37CC; 4. Aspirate and wash 3 times; 5. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37CC; 6. Aspirate and wash 5 times; 7. Add 100 uL Substrate Solution. Incubate 10 minutes at 37CC; 8. Read RLU value immediately.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance
Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96T
Available: In stock

Delivery expected until 6/27/2024 


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