Human Cartilage Intermediate Layer Protein 2 (CILP2) ELISA Kit (CLIA)

96-Well Plate

Tissue homogenates, cell lysates and other biological fluids

10.1 pg/mL

Chemiluminescent

Double-antibody Sandwich

Short term: 4CC; Long term: see manual.

8 months

This assay has high sensitivity and excellent specificity for detection of Cartilage Intermediate Layer Protein 2 (CILP2).

The microplate provided in this kit has been pre-coated with an antibody specific to Cartilage Intermediate Layer Protein 2 (CILP2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cartilage Intermediate Layer Protein 2 (CILP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cartilage Intermediate Layer Protein 2 (CILP2) level in the sample or standard.;

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.