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Human CTGF ELISA Kit PicoKine®

Human CTGF ELISA Kit PicoKine®

Item no.	BOS-EK2149
Manufacturer	Boster
Amount	96 wells/kit, with removable strips.
Category	Oncology By Cell Type Hematology Lymphoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	CCN2
Manufacturer - Specificity	Natural and recombinant human CCN2
Background	The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
Immunogen	Expression system for standard: NS0; Immunogen sequence: Q27-A349
Description	Human CTGF ELISA Kit PicoKine® (96 Tests). Quantitate Human CTGF in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA)
Sample Data	Concentration (pg/ml) \| 0 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1, 000 \| 2, 000 O.D. \| 0.016 \| 0.033 \| 0.065 \| 0.122 \| 0.289 \| 0.594 \| 1.052 \| 1.887
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean(pg/ml) \| 41 \| 153 \| 695 \| 45 \| 166 \| 726 Standard deviation \| 2.3 \| 8.72 \| 34.06 \| 2.75 \| 9.13 \| 28.31 CV(%) \| 5.6% \| 5.7% \| 4.9% \| 6.1% \| 5.5% \| 3.9%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 41 \| 61 \| 48 \| 55 \| 51 \| 1.85 \| 3.6% Sample 2 \| 153 \| 167 \| 185 \| 172 \| 169 \| 6.77 \| 4% Sample 3 \| 695 \| 701 \| 755 \| 786 \| 734 \| 38.18 \| 5.2% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from mouse\|biotinylated polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-EK2149-datasheet.pdf

BOS-EK2149-safety-datasheet.pdf