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Human Angiogenin/ANG PicoKineÂ® Quick ELISA Kit

Human Angiogenin/ANG PicoKineÂ® Quick ELISA Kit

Item no.

BOS-FEK0305

Manufacturer

Boster

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Human (Homo sapiens)

Sensitivity

<12 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Angiogenin|ALS9; ANG; Angiogenin; angiogenin, ribonuclease, RNase A family, 5; EC 3.1.27; EC 3.1.27.-; epididymis luminal protein 168; HEL168; MGC22466; MGC71966; Ribonuclease 5; RNase 5; RNASE5RNASE4

Available

Specificity

Natural and recombinant Human ANG

Manufacturer - Category

Fast ELISA Kits

Storage Conditions

Store at 4Â°C for 6 months, at -20Â°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Manufacturer - Gene Name

CST3

Gene Full Name

angiogenin, ribonuclease, RNase A family, 5

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Sample Data

Concentration (pg/ml)

Manufacturer - Specificity

Natural and recombinant Human ANG

Intra Assay Consistency & Inter Assay Consistency

Intra-Assay Precision

Inter-Assay Precision

Sample

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity\|Volume Anti-tag Pre-coated 96-well Strip Microplate\|1\|12 strips of 8 wells Standard\|\|2 Antibody A\|1\|3ml Antibody B\|1\|3ml Sample Diluent\|1\|15ml TBS-T Wash Buffer (25x)\|1\|12ml Color Developing Reagent (TMB)\|1\|10ml Stop Solution\|1\|10ml Adhesive Plate Sealers\|2\|Piece
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Background	Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: Q25-P147
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 Âµl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Manufacturer - Research Category	Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
Protein Name	Angiogenin
Description	Human Angiogenin/ANG PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Human Angiogenin/ANG PicoKineÂ® Quick ELISA Kit

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-FEK0305-datasheet.pdf

BOS-FEK0305-safety-datasheet.pdf