Rabbit SAA(Serum Amyloid A) ELISA Kit

Competitive Inhibition

Infection immunity; Kidney biomarker; Hepatology; Urology

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Serum Amyloid A (SAA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Serum Amyloid A (SAA) and unlabeled Serum Amyloid A (SAA) (Standards or samples) with the pre-coated antibody specific to Serum Amyloid A (SAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Serum Amyloid A (SAA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Serum Amyloid A (SAA) in the sample.