KIM1 BioAssay™ ELISA Kit, Human

Kits and Assays / Kits-ELISA, BioAssay™

4°C/-20°C

Sandwich ELISA quantitative detection kit for Human KIM1 in cell culture supernates, serum, plasma (heparin, EDTA) and urine.

KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rats is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to Rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the Rat Tim1 gene and database analysis, the HAVCR1 gene was mapped to 5q33.2.

Assay Range:
31.2pg/ml-2000pg/ml

Sensitivity:
< 2 pg/ml

Crossreactivity:
There is no detectable cross-reactivity with other relevant proteins.

Uniprot ID:
Q96D42

Entrez Gene ID:
26762

Kit Components:
96-well plate pre-coated with anti-KIM1, Human
Lyophilized recombinant KIM1, Human standard, 10ng/tube, 2Ea
USB Cat No Kit Component
143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)
143697 Sample diluent buffer, 30ml
143698 Antibody diluent buffer, 12ml
143699 ABC diluent buffer, 12ml
143700 TMB color developing agent, 10ml
143701 TMB stop solution, 10ml

Storage and Handling:
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.