KRISHZYME™ Enoxaparin Factor IIa Assay Kit

2 - 8°C

Chromogenic assay for testing Enoxaparin in purified systems by measurement of
factor IIa inhibition, in compliance with pharmacopoeias (EP/BP) and FDA guidelines

Intended Use:
Enoxaparin Factor lla is a chromogenic assay intended for the quantitative determination of enoxaparin in purified
solutions by measurement of factor IIa inhibition activity. The kit can be used for 100 test reactions as per
microtiter plate protocol.

Principle:
The inhibitory effect of anti-thrombin III (AT-III) on thrombin, factor IIa and other coagulation serine proteases in
plasma is increased several thousand-fold by enoxaparin. This inhibition accounts for the anticoagulant effect of
enoxaparin. The quantitative determination of enoxaparin levels by the measurement of their anti-IIa activity is a
necessary tool for monitoring treatment efficacy.
Presence of Enoxaparin catalyzes the reaction between AT lll and α-Thrombin. The factor IIa inhibition test is the
most useful assay covering the widest variety of enoxaparin preparations. In the assay, the rate of factor IIa
inhibition is directly proportional to the enoxaparin concentration since both factor IIa and AT-III are in excess. The
residual factor IIa activity is inversely proportional to the enoxaparin concentration.