KRIBIOLISA™ FSH (Follicle Stimulating Hormone) Bioassay ELISA

2 - 8°C

This KRIBIOLISA FSH (Follicle Stimulating Hormone) Bioassay ELISA is intended for determination of Human Follicle Stimulating Hormone (hFSH) in human serum

Introduction:
Human follicle stimulating hormone (FSH) is a glycoprotein hormone produced by the anterior pituitary gland.
There are three other glycoprotein hormones, namely Thyroid Stimulating Hormone, Luteinizing Hormone (both
produced by anterior pituitary gland) and Human Chorionic Gonadotropin (produced by the placenta) which are
structurally similar. Each hormone has an alpha and beta subunit. The α subunits of each hormone are similar
while the β subunit is specific to each hormone. The α subunits contain 92 amino acids while the β subunits vary
with each hormone. The β subunit of both FSH and LH contain 115 amino acids, TSH 110 amino acids, and hCG
147 amino acids. The FSH and LH hormones function differently in females and males. It is to be noted that in
women the growth and maturation of the ovarian follicle is dependent on FSH, while in men both LH and FSH act
on the testes.

Intended Use:
This kit is intended for determination of Human Follicle Stimulating Hormone (hFSH) in human serum.

Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to FSH are precoated onto microwells. Samples and standards are pipetted into microwells and anti-FSH antibodies linked to
HRP is added to the wells. FSH present in the sample and standards will bind to the plate and HRP Conjugate
simultaneously. Washing is done to remove the unbound samples, standards and HRP conjugate. The ready to
use substrate solution (TMB) is added to microwells and color develops directly proportional to the amount of
FSH in the standards and sample. Color development is then stopped by addition of stop solution. Absorbance is
measured at 450 nm.