KRIBIOLISA™ hCG (human Chorionic Gonadotropin) Bioassay ELISA

2 - 8°C

This KRIBIOLISA hCG (human Chorionic Gonadotropin) Bioassay ELISA is intended for determination of Human Chorionic Gonadotropin Hormone (hCG) in human serum

Introduction:
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by the placenta. During normal
pregnancy the level of this hormone in serum and urine gradually increases up to about the eighth week of
pregnancy. hCG has two subunits, namely α and β. The α-subunit is similar to the α-subunit of the anterior
pituitary gland glycoprotein hormones, namely, LH, TSH and FSH. In normal pregnancy the increase in hCG
starts at about the 5th day after conception and continues to rise until it reaches a maximum at about the eighth
week. It is a well known fact that hCG is also a tumour marker which is very important in the diagnosis of
choriocarcinoma.

Intended Use:
This kit is intended for determination of Human Chorionic Gonadotropin Hormone (hCG) in human serum.

Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to hCG are precoated onto microwells. Samples and standards are pipetted into microwells and anti hCG antibodies linked to
HRP is added to the wells. hCG present in the sample and standards will bind to the plate and HRP Conjugate
simultaneously. Washing is done to remove the unbound samples, standards and HRP conjugate. The ready to
use substrate solution (TMB) is added to microwells and color develops directly proportional to the amount of
hCG in the standards and sample. Color development is then stopped by addition of stop solution. Absorbance is
measured at 450 nm.
Please note that a two-step procedure is included and is to be used for assaying serum from pregnant women.