KRIBIOLISA™ VIP (H,B,P,R) (Aviptadil) ELISA

2 - 8°C

Immunoassay for quantitative estimation of VIP H-B-P-R in human serum, plasma and tissue extracts

Introduction:
VIP (Vasoactive intestinal peptide) is a peptide hormone that is vasoactive in the intestine. VIP is a peptide of
28 amino acid residues that belongs to a glucagon/secretin superfamily, the ligand of class II G protein–
coupled receptors. VIP is produced in many tissues of vertebrates including the gut, pancreas, and
suprachiasmatic nuclei of the hypothalamus in the brain. VIP stimulates contractility in the heart, causes
vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of
trachea, stomach and gall bladder. In humans, the vasoactive intestinal peptide is encoded by the VIP gene.

Intended Use:
The VIP-H-B-P-R ELISA is used for estimation of VIP-H-B-P-R in serum, plasma, or tissue extracts in
pharmacokinetics, peptide delivery study and other purposes.

Principle:
The VIP-H-B-P-R ELISA is a competitive immunoassay for the determination of VIP-H-B-P-R. The antiserum
is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and
varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum.
Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase),
which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by
dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution
from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount
of specific VIP-H-B-P-R bound.