Unfractionated Heparin (UFH) Ultra Range ELISA for Buffer

Heparin is a glycosaminoglycan with alternating uronic acid and aminoglycoside units. It is an anticoagulant used either in its native unfractionated form (UFH) MW ~, 16 kD or in various partially depolymerized forms (LMWH) of 4-8 kD. The heparin-ELISA product number K-2300 is a quantitative enzyme-linked assay designed for the in vitro measurement of unfractionated heparin levels in low protein content fluids such as buffer or urine. This assay measures heparin directly using a heparin binding protein which has been conjugated to HRP. The heparin-ELISA is a competitive assay in which the colorimetric signal is inversely proportional to the amount of heparin present in the sample. Samples to be assayed are first mixed with the Detector-Enzyme Conjugate in wells of the heparin coated plate. Heparin in the sample competes with heparin bound to the plate for binding of the Detector-Enzyme Conjugate. The concentration of heparin in the sample is determined using a standard curve of known amounts of heparin. For best results, the heparin used for the standard curve should match the type of heparin being assayed.

Multichannel Pipetter Absorbance microplate reader Plate Cover UFH Standard from USP reference or your heparin Standard Diluent, normal saline (154 mM NaCl)