Heparan Sulfate ELISA for Buffer

Dermatan sulfate (DS) is in the family glycosaminoglycan. DS is found mostly in the skin but also in blood vessels, tendons, heart valves, and pulmonary connective tissue. Heparan sulfate (HS)is a sulfated polysaccharide that is found on the surface of most cells as part of proteoglycans. Heparan sulfate is also present in the extracellular matrix. The polysaccharide mediates the interactions between a number of different proteins. The DS / HS ELISA, product number K-5800, is a quantitative enzyme-linked assay designed for the in vitro measurement DS / HS levels in buffer. This assay measures DS / HS directly using a DS / HS binding protein which has been conjugated to HRP. The DS / HS -ELISA is a competitive assay in which the colorimetric signal is inversely proportional to the amount of DS / HS present in the sample. Samples to be assayed are first mixed with the detector-enzyme conjugate in wells of the coated plate. DS / HS in the sample competes with DS / HS bound to the plate for binding of the detector-enzyme conjugate. The concentration of DS / HS in the sample is determined using a standard curve of known amounts of DS / HS.

Pipettes (8-channel multipipettor recommended) Absorbance microplate reader Dermatan / heparan sulfate standards Normal Saline (154 mM NaCl) Plate Cover