FASTKIT ELISA Ver. II BUCKWHEAT

ELISA

Buckwheat proteins

0.78 -50 ng / mL

1. Antibody immobilized plate (with cover) 96 wells (8 wells x 12 strips) x 1
2. Standard solution (50 ng/mL) 1.8 mL x 1
3. Dilution buffer 100 mL x 1
4. Biotin-labeled antibody 150 uL x 1
5. Enzyme (peroxidase)-labeled streptavidin 150 uL x 1
6. Chromogenic substrate (TMB) 12 mL x 1
7. Stop solution (0.5 N H2SO4) 12 mL x 1
8. Concentrated washing solution (1/10 concentration) 100 mL x 1
9. Extraction reagent 1 (1/20 concentration) 50 mL x 1
10. Extraction reagent 2 (1/20 concentration) 50 mL x 1
11. Extraction reagent 3 (1/20 concentration) 50 mL x 1
12. Instruction manual

[Supplementary]
Sandwich
[Applicable sample]
Food
[Other]
With the amendment of Food Sanitation Law-related laws and regulations in April 2002, food manufacturers are now required to specify proteins derived from the designated five raw materials considered likely to induce food allergy (egg, milk, wheat, buckwheat, and peanut) on the product label for all food products containing at least a few ug/mL or ug/g of such proteins. To provide accurate protein information, it is important to perform an assay for the designated raw materials to confirm the content of protein and exclude possible raw material derived carry-over or unexpected contamination during the manufacturing process.
This product is an enzyme-linked immunosorbent assay (ELISA)-based measurement kit for buckwheat protein in foods. It has been confirmed to meet the ''Guidelines for Evaluating Test Methods for Food Products Containing Allergic Substances'' as specified in the PFSB/DFS Notification No. 1106001 fromthe Director of the Dept. of Food Safety, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare dated November 6, 2002, ''Test methods for food products containing allergic substances'' (partially amended according to the PFSB/DFS Notification No. 1011002 dated October 11, 2005, PFSB/DFS Notification No. 0324001 dated March 24, 2006, and PFSB/DFS Notification No. 0622003 dated June 22, 2006 from the director of the same department).