Comparison

alpha-fetoprotein / AFP ELISA Kit, Human

Item no. OCE-EK1175
Manufacturer Bio-Ocean
Amount 96T
Category
Type Elisa-Kit
Applications ELISA
Specific against other
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Description
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human AFP. The Human alpha-fetoprotein/AFP ELISA is for research use only. Not for diagnostic or therapeutic procedures. alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal development. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. AFP is measured in pregnant women through the analysis of maternal blood or amniotic fluid, as a screening test for a subset of developmental abnormalities. AFP will be elevated in some of the diseases, including omphalocele, hepatocellular carcinoma/hepatoma, neural tube defects, nonseminomatous germ cell tumors, yolk sac tumor, ataxia telangiectasia.
Components
96-well polystyrene microplate coated with a monoclonal antibody against AFP
Human AFP Standard, lyophilized
AFP Detect Antibody
Assay Buffer (10x)
Substrate (TMB)
Stop Solution
Washing Buffer (20x)
Plate Covers
Assay Type
Sandwich ELISA
Cross-Reactivity
Human
Suitable Sample Type
serum, plasma, cell culture supernates
Format
96-well strip plate
Storage
4C (unopened)
standard stored at -20C, others stored at 4C (opened)
Sample Volume
100 ul (prediluted)
Sensitivity
0.45 pg/ml
Standard Curve Range
31.25 - 2000 pg/ml
Spike Recovery Range
90% - 110%
Mean Spike Recovery
0, 98
Range of Sample Value of Normal Sera
0.6 - 3.5 ng/ml
Detectable percentage of Normal Sera
100 % (n=30)
CV of Intra plate
1.6 % - 2.2 %
CV of Inter plate
1.6 % - 3.3 %

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96T
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close