Human LDL Receptor/LDLR ELISA Kit

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human LDLR. The Human LDL Receptor/LDLR ELISA is for research use only. Not for diagnostic or therapeutic procedures. Low-density lipoprotein (LDL) receptor (LDLR) is a mosaic protein of 839 amino acids which belongs to the LDLR gene family. It is a cell-surface receptor that recognizes the Apo B100, which is embedded in the outer phospholipid layer of LDL particles. The receptor also recognizes the Apo E protein found in chylomicron remnants and VLDL remnants. LDLR mediates the endocytosis of cholesterol-rich LDL. LDLR complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells, but mainly in the liver which removes ca.70% of LDL from the circulation. Hyperthyroidism may be associated with hypocholesterolaemia via upregulation of the LDLR, and hypothyroidism with the converse.

Sandwich ELISA

serum, plasma, cell culture supernates

4C (unopened)
standard stored at -20C, others stored at 4C (opened)

6.32 pg/ml

62.2 - 184.1 ng/ml

3.0 % - 4.3 %