Human sIL-2Ralpha/CD25 ELISA Kit

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human sIL-2Ralpha/CD25. The Human sIL-2Ralpha/CD25 ELISA is for research use only. Not for diagnostic or therapeutic procedures. The interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to a cytokine called IL-2. IL-2R is consist of three chains, an alpha chain (IL-2Ralpha, CD25), a beta chain (IL-2Rbeta, CD122) and a gamma chain (IL-2Rgamma, CD132). The alpha chain binds IL-2 with low affinity, the combination of beta and gamma together form a complex that binds IL-2 with intermediate affinity, primarily on memory T cells and NK cells. A model of the IL-2R complex would describe the high affinity receptor as an alphabetagamma trimer, in which all three chains are in contact with the ligand. IL-2 and its receptor have key roles in key functions of the immune system, tolerance and immunity, primarily via their direct effects on T cells. But the function of the soluble IL-2 Ralpha (sIL-2Ralpha) is unclear, since it would be expected to be a poor inhibitor of IL-2 because of its low binding affinity. In any case, increased levels of the sIL-2Ralpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Monitoring of sIL-2Ralpha levels in serum provides more detailed insights in several pathological situations such as rheumatoid arthritis, systemic lupus erythematosis (SLE), some leukemias and lymphomas.

Sandwich ELISA

serum, plasma, cell culture supernates

4C (unopened)
standard stored at -20C, others stored at 4C (opened)

2.99 pg/ml

93 % - 112 %

356.8 - 1275.8 pg/ml

4.8 % - 7.5 %