IL-1beta Pro-form ELISA Kit, Mouse

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-1beta Pro-form. The Mouse IL-1beta Pro-form ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin-1 beta (IL-1beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1beta into the active 17 kDa protein requires the activation of inflammasomes. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1beta. Although IL-1beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1beta signals through two receptors, IL-1RI and IL-1RII. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1beta neutralizing antibodies potential therapeutical value.

Sandwich ELISA

serum, plasma, cell culture supernates

4C (unopened)
standard stored at -20C, others stored at 4C (opened)

1.98 pg/ml

83 % - 111 %

n.d.

1.9 % - 4.4 %