Rat TNF-alpha ELISA Kit

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat TNF-alpha. The Rat TNF-alpha ELISA is for research use only. Not for diagnostic or therapeutic procedures. Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF1A, is an adipokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha may play a significant role in the pathogenesis of inflammatory disease of the joints and other tissues. Recently, a growing body of information has pointed to a role for TNF-alpha in the pathogenesis of AIDS. Measurement of TNF-alpha levels has also been shown to be useful in transplant research.

Sandwich ELISA

serum, plasma, cell culture supernates

4C (unopened)
standard stored at -20C, others stored at 4C (opened)

7.14 pg/ml

82 % - 116 %

n.d. - 132.8 pg./ml

4.4 % - 6.3 %