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Albuwell Hu

Albuwell Hu

Item no.	1004
Manufacturer	Exocell
Amount	96 wells
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	Albuwell II
Available
Manufacturer - Category	Albumin ELISA
Expected Values	A total of 42 time urine samples representing collection intervals from 2-8 hours were collected from normal volunteers. Normal excretion rate ranged from 1.7-16.1 ug/min., with an average of 4.3 ug/min. 95% of the normal population had excretion rates less than 9.5 ug/min. A random screening of diabetic patients showed that 16 (51%) had normal levels of urinary albumin, 10 (33%) were microalbuminuric and 5 (16%) were proteinuric.
Methodology	Competitive ELISA
Precision	Intra- and interassay precision for samples within the assay range have a C.V.<7%.
Summary of procedure	These kits were designed to monitor kidney function by quantitative estimation of albumin in human urine. They successfully measure albumin concentrations well outside the range of conventional dip-stick methods. To complete the assay, standard dilutions and urine samples are added to a plate precoated with human albumin. An enzyme conjugated anti-albumin antibody is added, and the plate is incubated for 30 minutes. During this time the conjugated antibody binds to either the albumin on the solid phase or to that in the liquid phase, hence the notion of competition. Thereafter, components remaining in the liquid phase are washed away, and bound conjugate determined using TMB Substrate Solution. The chromogenic reaction is stopped after 2-5 minutes using dilute acid (Albuwell). Color intensity is determined spectrophotometrically, with a microtiter plate reader (Albuwell).
Specificity	The antibody is specific for human albumin. It does not cross-react with rat albumin or with other proteins that occur in urine.
Method Comparison	Diagnostic Products Corp., Double Antibody RIA

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