Blood Genomic DNA Extraction MAXI Kit (10prep) (With Proteinase K Powder, for wide applications and simple procedure)

1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
2. Preheat a thermal incubator to 60 C before the operation.
3. Use a centrifuge with a swinging bucket rotor and a force of 4, 000 ~ 6, 000 x g for in all centrifugation steps.
4. Add 500 ul of sterile ddH2O to proteniase K tube to make a 22 mg/ ml stock solution. Vortex and make sure
that Proteinase K powder has been completely dissolved. Store the stock solution at 4 C.
5. Perheat the Elution Buffer or ddH2O for step 11 (Elution step).

up to 10 ml of fresh/ frozen blood;
up to 1 x 10 of cultured cells

1 hour

500 ug of DNA