Circulating Nuleic Acid Isolation Kit

All buffers should be stored at room temperature (15-25C). Lyophilized Proteinase K should be stored at a€" 20C and prepared Proteinase K solution should be stored at 2a€" 8C. Lyophilized Carrier RNA should be stored at a€" 20C.

1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling
these buffers.
2. Make sure everything is RNase-free when handling RNA.
3. CAUTION: Buffers CL, CB and CW1 contain guanidinium salts which can form highly reactive
compounds when combined with bleach. DO NOT add bleach or acidic solutions directly
to the preparation waste.
3. Add CE Elution Buffer to lyophilized Proteinase K to make a 10 mg/ml stock solution. Vortex
and make sure that Proteinase K has been completely dissolved. Store the stock solution
at 4 C.
4. Add CE Elution Buffer to lyophilized Carrier RNA then store at a€" 20 C. Do not freezea€"thaw the
Carrier RNA solution more than three times.
5. Add isopropanol to CB Binding Buffer. And add ethanol to CW1 Wash Buffer and CW2
Wash Buffer when first use.
6. All centrifugation steps are done at full speed (~ 18, 000 x g) at room temperature.
7. This kit is suitable for the isolation of nuceic acid from fresh or frozen serum/ plasma prepared
from blood collected on Heparin, EDTA or citrate.
8. Make sure the plasma or serum samples are clear. Centrifuge the samples for 2 minutes
at 400 x g if the debris are still visible.

mini spin column (silica matrix)

40 ul