Food DNA Extraction Kit I(50prep)

1. Homogenize the starting sample completely with a proper homogenizer and mix the sample powder evenly before the
DNA extraction.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
3. Store the Proteinase K tube at 4 C on upon receipt of kit. And dissolve the Proteinase K with ddH2O to make a 20 mg/ml
stock solution and store the stock at 4 C.
4. Check Lysis Buffer before use, Warm Lysis Buffer at 60C for 5 minutes if any precipitate formd.
5. Preheat shaking incubator to 60CC before the operation.
6. Preheat Elution Buffer to 60 C for Elution step.
7. Add required ethanol (96-100%) to Wash Buffer before use.

spin column (silica membrane)

1. Homogenizer
2. Centrifuge tube and centrifuge:
For 2 g starting sample: 50 ml centrifuge tube and centrifuge with rotor for 50 ml tube, microcentrifuge tubes (1.5 ml and
2.0 ml) and microcentrifuge.
For 200 mg starting sample: microcentrifuge tubes (1.5 ml and 2.0 ml) and microcentrifuge.
3. Ethanol (96 ~ 100 %)
4. Chloroform
5. Shaking incubator (60 C)
6. Vortexer
7. Pipets and pipet tips