| Background |
The included ELISA 96-well micro-strip plate is provided pre-coated with an affinity purified rabbit polyclonal antibody selective to the antigen. Upon addition of samples to the wells, the antigen in solution binds to the antibody and becomes indirectly linked to the solid support. After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period. Unbound antibody is washed, and then color reagents are added, which upon conversion by HRP, become blue colored. A stop solution is added to block further reaction between HRP and the colorimetric substrates, converting the solution into a yellow coloration. An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm. This assay requires approximately 1.5 hrs to complete at 37C. |
| References |
Xabier Agirre, et.al. (2006). Abnormal methylation of the common PARK2 and PACRG promoter is associated with downregulation of gene expression in acute lymphoblastic leukemia and chronic myeloid leukemia. International journal of cancer Journal international du cancer 118 (8) p. 1945-1953.
Nonmotor Symptoms in Patients with PARK2 MutationsAsako Yoritaka, Yumi Shimo, Yasushi Shimo, Yuichi Inoue, Hiroyo Yoshino, Nobutaka Hattori (2011). Parkinsons disease 2011 p. 47364.
George Poulogiannis, Rebecca E McIntyre, Maria Dimitriadi, John R Apps, Catherine H Wilson, Koichi Ichimura, Feijun Luo, Lewis C Cantley, Andrew H Wyllie, David J Adams, Mark J Arends (2010). PARK2 deletions occur frequently in sporadic colorectal cancer and accelerate adenoma development in Apc mutant mice.
Proceedings of the National Academy of Sciences of the United States of America 107 (34) p. 15145-15150.
Yasuhiro Yamamura (2007). The long journey to the discovery of PARK2. Rinsho shinkeigaku Clinical neurology 47 (11) p. 749-751. |
