| Highlights |
Sandwich ELISA format providing convenience, high sensitivity and selectivity
Highly sensitive; pg/ml detection
Affinity purified polyclonal capture antibody coated on plate. Separate HRP-conjuated detection antibody
Rapid colorimetric detection. 1 hr total incubation at 37C; 2 hr incubation at RT
Suitable for membrane extracts, cell lysates, tissue homogenates and other mediums |
| Background |
Presenilins are a family of related multi-pass transmembrane proteins that function as a part of the gamma-secretase protease complex. Vertebrates have two presenilin genes, called PSEN1 (located on chromosome 14 in humans) that encode Presenilin 1 (PS-1) and PSEN2 (on chromosome 1 in humans) that codes for presenilin 2 (PS-2). Both genes show conservation between species, with little difference between rat and human presenilins.
Presenilins undergo cleavage in an alpha helical region of one of the cytoplasmic loops to produce a larger N-terminal and a smaller C-terminal fragment, which together form part of the functional protein. Cleavage of presenilin 1 can be prevented by a mutation which causes the loss of exon 9, and results in loss of function. Presenilins play a key role in the modulation of intracellular Ca2+ involved in presynaptic neurotransmitter release and long-term potentiation induction.
Dominant mutations in the genes that encode presenilin proteins are the most common cause of familial early-onset Alzheimer's disease. |
| References |
Gamliel A, Teicher C, Michaelson DM, Pradier L, Hartmann T, Beyreuther K, Stein R. Increased expression of presenilin 2 inhibits protein synthesis. Molecular And Cellular Neurosciences 19: 111-124, 2002.
Kim TW, Pettingell WH, Hallmark OG, Moir RD, Wasco W, Tanzi RE. Endoproteolytic cleavage and proteasomal degradation of presenilin 2 in transfected cells. The Journal of Biological Chemistry 272: 11006-11010, 1997.
Takahashi H, Mercken M, Nakazato Y, Noguchi K, Murayama M, Imahori K, Takashima A. Cloning of cDNA and expression of the gene encoding rat presenilin-2. Gene 197: 383-387, 1997.
Tanimukai H, Tsujio I, Hashimoto R, Kudo T, Kamino K, Shinozaki K, Takeda M. Presenilin-2 mutation and polymorphism in Japanese Alzheimer disease patients. Clinica chimica acta international journal of clinical chemistry 283: 57-61, 1999. |
