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Ubiquitin Conjuating Enzyme (E2/UBCE) ELISA Kit

Ubiquitin Conjuating Enzyme (E2/UBCE) ELISA Kit

Item no.	hE2UBCE-ELISA
Manufacturer	Fivephoton
Amount	1 kit, 96 T
Category	Cell Biology Cell Status ELISA Autophagy ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Citations	1. Wiebel, Franziska F., and Wolf-H. Kunau. 1992. "The Pas2 protein essential for peroxisome biogenesis is related to ubiquitin conjugating enzymes." Nature 359.6390, 73-76. 2. Seufert, Wolfgang, Bruce Futcher, and Stefan Jentsch.1995. "Role of a ubiquitin-conjugating enzyme in degradation of S-and M-phase cyclins." 78-81. 3. Jahngen-Hodge, Jessica, et al. 1997. "Regulation of ubiquitin-conjugating enzymes by glutathione following oxidative stress." Journal of Biological Chemistry 272.45, 28218-28226. 4. Moraes, Trevor F., et al. 2001. "Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13." Nature Structural & Molecular Biology 8.8, 669-673.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Hazard information	Thimerosal
Available
Storage Conditions	4°C
Poisonous Material	Thimerosal
Background	Ubiquitin-conjugating enzymes are known as E2 enzymes. They perform the second step of ubiquitination. The E2 enzyme receives activated ubiquitin from E1, conjugates it, then binds to E3 where it is transferred to a lysine residue on the targeted protein. The overall ubiquitination reaction causes protein degradation by proteasomes. Ubiquitin conjugating enyme is located in the cytosol. To isolate fractions for ELISA assay, use a non-denaturing detergent lysis buffer such as Part No. ELSP-1, or similar to lyze membranes and release a soluble cytosolic fraction. The protocol associated with Part No ELSP-1 can be used to isolate cytosolic fractions for ELISA measurement.
Highlights	Pre-configured ELISA kit in sandwich assay format. Dynamic Assay Range: 100pg/ml - 30ng/ml. Sensitivity: 37pg/ml. Standard peptide concentration: 32ng/ml Rapid and simple ELISA Protocol: Requires one 60 min incubation period at 37C. Samples are mixed with biotinylated detection antibody and streptavidin-HRP prior to addition to the plate. Available in 96T format.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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hE2UBCE-ELISA-safety-datasheet.pdf