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Ubiquitin Ligase (E3) ELISA Kit

Ubiquitin Ligase (E3) ELISA Kit

Item no.	hE3UBPL-ELISA
Manufacturer	Fivephoton
Amount	1 kit, 96 T
Category	Neuroscience Neuroinflammation Neural Mitochondrial Function Protein Aggregation, Clearance & Misfolding Cell Biology Cell Status ELISA Autophagy ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Human (Homo sapiens)
Citations	1. Robinson, P. A., and H. C. Ardley. 2004. "Ubiquitin-protein ligases." Journal of cell science 117.22, 5191-5194. 2. Shimura, Hideki, et al. 2000. "Familial Parkinson disease gene product, parkin, is a ubiquitin-protein ligase." Nature genetics 25.3, 302-305. 3. Yang, Yili, et al. 2000. "Ubiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli." Science 288.5467, 874-877. 4. Hershko, AHHESaCA, et al. 1983. "Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown." Journal of Biological Chemistry 258.13, 8206-8214.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Hazard information	Thimerosal
Available
Storage Conditions	4°C
Poisonous Material	Thimerosal
Background	Ubiquitin ligase is the last enzyme in the cascade involved in the conjuation of ubiqutin to lysine. It operates in a complex with E2 ubiqutin acitvating enzyme. which is located in the cytosol, and attaches ubiquitin to lysine thorugh an isopeptide bond. The E3 enzyme is involved in the attachment of polyubiqutiin that targets proteins to degradation in the proteasome. The ubiqitin conjugating machinery is cytoplasmic and can be extracted for ELISA using a non-denaturing cell lysis bufer such as Part ELSP-1, or similar that releases a soluble cell lysate. The protocol for Part ELSP-1 provides a method to isolate a fraction with E3 for ELISA analysis.
Highlights	Pre-configured ELISA kit in sandwich assay format. Dynamic Assay Range: 100pg/ml - 40ng/ml depending on species. Sensitivity: 42pg/ml. Standard peptide concentration: 40-48ng/ml depending on species. Rapid and simple ELISA Protocol: Requires one 60 min incubation period at 37C. Samples are mixed with biotinylated detection antibody and streptavidin-HRP prior to addition to the plate. Available in 96T format.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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hE3UBPL-ELISA-safety-datasheet.pdf