Apo E ELISA Kits (for human, mouse, rat)

4°C

ApoE transports lipoproteins, fat-soluble vitamins, and cholesterol into the lymph system and into the blood. It is synthesized in the classical secretory pathway principally in the liver and is also expressed in brain, kidney, and spleen. In the nervous system, non-neuronal cell types, most notably astroglia and microglia, are the primary producers of ApoE, while neurons express the ApoE receptors. There are seven currently identified mammalian receptors for ApoE.
ApoE has several major allelic isoforms: ApoE2, ApoE3, ApoE4: Normal ApoE-epsilon3: Dysfunctional ApoE-epsilon2 and ApoE-epsilon4. The major allelic forms differ by amino acid substitutions at positions 112 and 158. The E2 allele codes for Cys at positions 112 and 158 in the receptor-binding region. In contrast, the E3 allele expresses Cys-112 and Arg-158 at these positions, and the E4 allele has Arg at both positions.

E2 is associated with the genetic disorder hyperlipoproteinemia type III and atherosclerosis. E3 is found in approximately 64 percent of the population; it is considered the "neutral" Apo E genotype. E4 has been implicated in atherosclerosis and Alzheimer's disease, impaired cognitive function, and reduced neurite outgrowth.

Apo E Isolation for ELISA Assay:
Apo E undergoes synthesis, processing and secretion in the classical secretory pathway1. Therefore, it is located in compartments of the secretory pathway that include endoplasmic reticulum, Golgi and secretory vesicles, as well as the extracellular medium such as plasma and serum, and in cell culture medium. It can be isolated from intracellular compartments using detergent lysis and solubilization, or in the extracellular medium (that include plasma or cell culture medium) by sedimentation of cells and collection of the supernantant.