Mouse Rantes ELISA Kit

CCL5

This mouse Rantes ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Rantes has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rantes is added subsequently and then followed by washes with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is then added and unbound conjugates were washed away with PBS or TBS buffer. The HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the concentration of Rantes captured on the plate.