Mouse E-Cadherin ELISA Kit

CD324

This mouse E-Cadherin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for E-Cadherin has been precoated onto 96-well plates. Standards(from plasma) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Cadherin is added subsequently and then followed by washes with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates were washed away with PBS or TBS buffer. The HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow is proportional to the E-cadherin concentration captured on the plate.