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Insulin Degrading Enzyme ELISA Kit Format 1 (mouse)

Insulin Degrading Enzyme ELISA Kit Format 1 (mouse)

Item no.	mIDE-Biotin
Manufacturer	Fivephoton
Amount	96 T
Category	Neuroscience Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Protein Aggregation, Clearance & Misfolding ELISA Endocrine Regulation of Lipid Metabolism ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Citations	Amata, O., Marino, T., Russo, N., & Toscano, M. (2009). Human insulin-degrading enzyme working mechanism. Journal of the American Chemical Society, 131(41), 14804-14811. Bennett, R. G., Duckworth, W. C., & Hamel, F. G. (2000). Degradation of amylin by insulin-degrading enzyme. The Journal of Biological Chemistry, 275(47), 36621-36625. Grasso, G., Rizzarelli, E., & Spoto, G. (2008). How the binding and degrading capabilities of insulin degrading enzyme are affected by ubiquitin. Biochimica et Biophysica Acta, 1784(7-8), 1122-1126. Hong, M.-G., Reynolds, C., Gatz, M., Johansson, B., Palmer, J. C., Gu, H. F., Blennow, K., et al. (2008). Evidence that the gene encoding insulin degrading enzyme influences human lifespan. Human Molecular Genetics, 17(15), 2370-2378. Oxford University Press. Im, H., Manolopoulou, M., Malito, E., Shen, Y., Zhao, J., Neant-Fery, M., Sun, C.-Y., et al. (2007). Structure of substrate-free human insulin-degrading enzyme (IDE) and biophysical analysis of ATP-induced conformational switch of IDE. The Journal of Biological Chemistry,282(35), 25453-25463. Vepsalainen, S., Hiltunen, M., Helisalmi, S., Wang, J., Van Groen, T., Tanila, H., & Soininen, H. (2008). Increased expression of Abeta degrading enzyme IDE in the cortex of transgenic mice with Alzheimer’s disease-like neuropathology. Neuroscience Letters.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Available
Manufacturer - Targets	INS, IDE
Background	The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen. Samples are pre-mixed with a provided biotin-linked detection antibody and provided streptavidin-HRP, which are then added to the plate. The plate is washed after a 60 min incubation period. Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution. A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.
Highlights	Fully pre-configured ELISA kit in sandwich assay format. Dynamic Assay Range: 300pg/ml - 60ng/ml. Sensitivity: 226pg/ml. Rapid ELISA Protocol: Requires one 60 min incubation period at 37C. Samples are mixed with the provided biotinylated detection antibody and provided streptavidin-HRP prior to addition to plate. Available in 48T and 96T formats.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 T

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Delivery expected until 7/31/2025

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