Mouse Insulin Degrading Enzyme (IDE) ELISA Kit

Manufacturer Fivephoton
Category Products / ELISA
Type Elisa-Kit
Specific against Mouse
Amount 96T
ArtNr mIDE-Biotin
Eclass 6.1 32160605
Eclass 9.0 32160605
Available
Highlights
Fully pre-configured ELISA kit in sandwich assay format.
Dynamic Assay Range: 300pg/ml - 60ng/ml. Sensitivity: 226pg/ml.
Rapid ELISA Protocol: Requires one 60 min incubation period at 37C.
Samples are mixed with the provided biotinylated detection antibody and provided streptavidin-HRP prior to addition to plate.
Available in 48T and 96T formats.
Background
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen. Samples are pre-mixed with a provided biotin-linked detection antibody and provided streptavidin-HRP, which are then added to the plate. The plate is washed after a 60 min incubation period. Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution. A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.
References
Amata, O., Marino, T., Russo, N., & Toscano, M. (2009). Human insulin-degrading enzyme working mechanism. Journal of the American Chemical Society, 131(41), 14804-14811.

Bennett, R. G., Duckworth, W. C., & Hamel, F. G. (2000). Degradation of amylin by insulin-degrading enzyme. The Journal of Biological Chemistry, 275(47), 36621-36625.

Grasso, G., Rizzarelli, E., & Spoto, G. (2008). How the binding and degrading capabilities of insulin degrading enzyme are affected by ubiquitin. Biochimica et Biophysica Acta, 1784(7-8), 1122-1126.

Hong, M.-G., Reynolds, C., Gatz, M., Johansson, B., Palmer, J. C., Gu, H. F., Blennow, K., et al. (2008). Evidence that the gene encoding insulin degrading enzyme influences human lifespan. Human Molecular Genetics, 17(15), 2370-2378. Oxford University Press.

Im, H., Manolopoulou, M., Malito, E., Shen, Y., Zhao, J., Neant-Fery, M., Sun, C.-Y., et al. (2007). Structure of substrate-free human insulin-degrading enzyme (IDE) and biophysical analysis of ATP-induced conformational switch of IDE. The Journal of Biological Chemistry, 282(35), 25453-25463.

Vepsalainen, S., Hiltunen, M., Helisalmi, S., Wang, J., Van Groen, T., Tanila, H., & Soininen, H. (2008). Increased expression of Abeta degrading enzyme IDE in the cortex of transgenic mice with Alzheimer’s disease-like neuropathology. Neuroscience Letters.
Amount: 96T
Available: In stock
Listprice: €804.92
Discount: -10%
Price: €724.43
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